Plant
Propagation

Other
Services

Plant
Propagation

"The goal of In Vitro culture is to regenerate an entire plant from cells or vegetative tissues (explants) in a nutrient medium".

The Sampling

The choice of the explant to be taken is particularly important. For each species, are taken into account: the propagation technique envisaged, the date of sampling, the age of the mother’s foot, the nature, the size as well as the location of the exilant on the mother’s foot.

In Vitro Culture

The explants are then disinfected, worked under aseptic conditions, and placed on a sterile growth medium. This support includes foods: macro-elements (N, P, K, Ca, Mg, M, …), micro-elements (Bo, Fe, Cu, …), sugar, vitamins, growth regulators, (essentially auxins and cytokinins whose balance determines organogenesis) and an agar base that solidifies the culture medium. The composition of the medium and its pH are adapted to the species cultured. Each lot is identified to be traced: identity of the mother plant, date of cultivation, etc …

The multiplication

Strict control of the environment is necessary for the proper growth of vitroplants in an artificial environment. The parameters of hydrometry, temperature and luminosity (both intensity and duration day / night) must be adapted to each species worked. Each container (jar, box or tube) is labeled to ensure traceability.

Acclimation

After the initiation of the rooting phase in the laboratory, the aim is to acclimatize young vitroplants to the external environment. However, moving from air-conditioned laboratories to greenhouses requires an intermediate step of a few weeks during which the plant develops its root system. This phase of acclimatization takes place in specific premises (greenhouse No. 1) allowing precise control of lighting conditions, temperature and especially humidity.

At this stage, seedlings are housed in honeycomb plate. The substrates used are disinfected with steam to eliminate any pathogenic risk. This sterilization creates a microbiological vacuum favorable to a good installation of biotization: the non-benevolent microorganisms are replaced by micro-organisms beneficial to the development of the plant.

Growth Phase

After acclimatization, the plants are repotted in biodegradable bags and driven under greenhouses for a few months. They are fed by fertigation and are always monitored daily. At the end of this breeding period, the plants reached a general morphology and a height allowing them to be planted. That’s when they are delivered to our customers.

Important Note

As explained in this last point, some of our customers will choose to be delivered at the end of the breeding phase, while others will prefer to receive their plants after the acclimatization to achieve the phase of breeding. on-site breeding. It will also be common to deliver seedlings in a sterile environment at the end of the rooting stage, so that acclimatization and rearing are performed at our customers.

To remember

Our ability to adapt to your requirements, your wishes and your constraints.

Our job is to understand your project and your problems, to answer them with an optimal technical solution and a satisfactory price, then to deliver the products and services that will satisfy the highest levels of requirement.

For us, every customer is unique! And, obviously, we guarantee the exclusivity of the material provided by our customers, as well as the total confidentiality of the information transmitted.

"With our partnership in R & D with the biotechnology laboratory of the University of Ghent, we have access to multiplication know-how for a large number of plant species, spread over the main families of use".

Our offer in plant propagation:

3 big families, a multitude of achievable products...

Food

Food Plants

Forestry

Forestry Plants

Fun

Fun Plants

FOOD | Food Plants

Berries

Blueberry

Vaccinium myrtillus - corymbosum

Raspberry

Rubus idaeus

Blackberry

Rubus fructicosus

Black Currant

Ribes sp.

Dried Fruits

Pistachio

Pistacia vera

Hazelnut

Corylus avellana

Nut

Junglans regia

Almond

Prunus dulcis

Local Products

Coffee

Coffea arabica-canephora

Cacao

Theobroma cacao

Manioc

Manihot esculenta

Banana

Musa × paradisiaca

FORESTRY | Forestry Plants

Palisander

Dalbergia retusa

Guayacan

Tabebuia chrysantha

Local Mahogany

Swietenia macrophylla

Cuban Mahogany

Swietenia mahagoni

Paulownia

Paulownia sp.

Teak

Tectona grandis

FUN | Fun Plants

Anthurium

Anthurium Andreanum

Christ Crown

Euphorbia Milli

Bromeliad

Guzmania sp. - Thilandsia sp. - Vriesea sp.

Philodendron

Philodendron sp.

Pink Princess

Philodendron Pink Princess

Orchids

Phalaenopsis

Spathiphyllum

Spathiphyllum sp.

Medinilla

Medinilla magnifica

Other
Services

GreenLab Biotechnology offers the most advanced biotechnological services, so you can develop the products your business needs.

Sanitation of
plant strains

"Sanitation of strains makes it possible to put healthy plants back into the production circuit, thus limiting chemical treatments".

GREENLAB BIOTECHNOLOGY cleanses lines and varieties by: Meristem culture and thermotherapya.

Meristem culture

For most species, the meristemic tissue of the vegetative buds of the plant is removed for culturing. This tissue is free from viruses or bacterioses, even in infected plants. Formed of undifferentiated cells, this meristem can regenerate an entire plantlet (totipotence of plant cells). The resulting seedling is subsequently multiplied, always in vitro to ensure the perfect sanitary quality of the offspring.

Primary meristem

seen under a microscope

Thermotherapy

In other cases, sanitation may be coupled with thermotherapy, ie the use of heat for therapeutic purposes. A gradual and controlled rise in heat can weaken and then destroy the infected cells, while respecting healthy cells.

Cryopreservation

"The cryopreservation of plant fragments at very low temperatures in liquid nitrogen preserves genetic resources without genetic drift and for a practically indefinite duration".

Cryopreservation is the freezing of plant tissues at very low temperatures by the use of liquid nitrogen.

For a breeder wishing to save the cost of his work at a lower cost, a company wishing to preserve rare or precious plant material, or an individual attached to a remarkable plant, this technique is the ideal support. But keeping several hundred varieties for 10 or 20 years is complex. Cost, time, risks of genetic drift, health risks, accidental climatic risks: breeders retain only hybrids with short-term potential and must abandon potentially interesting species in the long term. However, cryopreservation offers the possibility of maintaining the biodiversity potential intact, thus the genetic diversity, sheltered from all external events for very long periods. Cryopreservation also makes it possible to carry out a retro-evaluation on the stored material, and to follow the evolution of a variety with respect to the initial cryopreserved strain. (appearance of chimeras or mutations, adaptation to the environment, etc…).

Inoculation by
micro-organisms

"The microflora is an indicator of the quality of a soil, that is, the balance between the plants, the soil and its micro-organisms. When this balance is threatened by cultural practices, the land loses its function of providing food and becomes a simple support".

GreenLab Biotechnology offers three original uses of microorganisms to optimize the resources of your soils and the potential of your plants:

Biotization
The detection of pathogenic microorganisms
Optimization of natural defense systems

Biotization (specialty of GreenLab Biotechnology)

In undisturbed soils, the plant benefits from soil fungi and bacteria through its root system. It is possible to search and select the most suitable micro-organisms, and to inoculate them to the plants to improve their vigor. This is particularly useful in agricultural soils or in horticultural substrates where populations of these micro-organisms are scarce or absent. These inoculated plants reduce inputs and return to a rational use of soil resources.

"This biotization technique is proving very effective in boosting the growth, vigor and robustness of many varieties, particularly in the forestry".

Detection of pathogenic micro-organisms

This involves performing visual checks by microscopy and molecular marker analyzes.

Optimization of natural defense systems

It involves selecting micro-organisms (fungi, bacteria, yeasts) or natural molecules (SDN) according to their potential for stimulating defense reactions. This alternative is the only operational solution for many minor species.

Genetic
Analysis

"Each variety is characterized by its molecular profile.The genetic fingerprint helps identify high value-added plants, and track the evolution of a variety from the original strain".

We offer you a full range of state-of-the-art analytical services, access to which remains confidential today:

Sanitary diagnosis of plants and soils
Identification of bacteria and fungi
Mycorrhizal control
Identification of mycorrhizal fungi
Varietal identification

Sanitary diagnosis of plants and soils

To limit the outbreaks of infections in your plantations, it is necessary to detect as early as possible and as quickly as possible plants with disease. It is also important to test the absence of pathogens in the soil before planting. While it is easy to diagnose most common infections, a large number of orphan diseases are complex to identify. GreenLab Biotechnology enables its customers to access modern phyto-diagnostic techniques based on DNA detection (PCR and sequencing) for the identification of pathogenic fungi and bacteria in plant tissues, culture media and soils. These tests include, for example, the detection of Phytophthora fragariae and Rhizoctonia.

Identification of bacteria and fungi

More generally, GreenLab Biotechnology offers you the identification of bacteria and fungi that interest you. Be it contaminating or beneficial microorganisms of various origins (nature, agriculture, agro-food, etc …). For this, we use molecular biology techniques based on PCR and sequencing.

Mycorrhizal control

Would you like to know the level of mycorrhization of your plants, whether inoculated or naturally mycorrhizal? GreenLab Biotechnology analyzes plant mycorrhization by microscopic observation. This includes endomycorrhizal herbs and ectomycorrhizal forest trees.

Identification of mycorrhizal fungi

GreenLab Biotechnology can identify mycorrhizal (endomycorrhizogenic or ectomycorrhizogenic) fungi present in roots or soil through morphological analysis supplemented by molecular analysis.

These tests are particularly interesting in truffle plantations to check the survival of truffle mushrooms against competition by other truffle or non-truffle ectomycorrhizal fungi (example: Tuber rufum or Sclerodema).

Varietal identification

We can perform on your behalf a specific genetic imprint of a plant species or variety. This becomes necessary to ensure the identification and protection of high value-added plants in cases of counterfeiting or geographical identification (PGI).

You can ask us to validate the varietal purity of your plant strains.

"In addition to the production of countless plant varieties to meet the most diverse and varied needs, GreenLab Biotechnology offers you a wide range of know-how to support you in your projects through our expertise, our technique, and our professionalism, we will bring you what you need.".

Plant Propagation

Other Services